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Basic Techniques in Biochemistry and Molecular Biology
Basic Techniques in Biochemistry and Molecular Biology

Tags: Basic Techniques in Biochemistry and Molecular Biology, Life Sciences, Biochemistry, R.K. Sharma, S.P.S. Sangha

Basic Techniques in Biochemistry and Molecular Biology

Fundamentals of biochemistry and molecular biology is an important component of all disciplines of Biology. In the era of multidisciplinary approach, the basic techniques in Biochemistry and Molecular Biology are much needed by the students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied subjects both at undergraduate and postgraduate levels. This book includes 15 chapters covering more than 135 experimental protocols. It discussed all the relevant topics like pH and buffers, spectrophotometry, chromatography, carbohydrates, lipids, proteins, electrophoresis, enzyme immunology, vitamins and pigments, metabolites and molecular biology. It includes a wide range of experiments from preparation of culture media to PCR, Southern and Western blotting. All the experiments have been meticulously designed and special care has been taken to the safety in laboratory and precautions are given wheresoever required.
1.General Introducton 
2.pH and Buffers 
3.Spectrophotometry 
4.Centrifugation and Cell Fractionation 
5.Chromatography 
6.Carbohydrates 
7.Lipids 
8.Amino Acids and Proteins 
9.Nucleic Acids 
10.Electrophoresis 
11.Immunology 
12.Enzymes 
13.Metabolites 
14.Vitamins, Hormones and Pigments 
15.Molecular Biology 
16.Appendices Readership: Students of Botany, Zoology, Microbiology, Biotechnology,
  Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied
  subjects both at undergraduate and postgraduate levels.
  • Spread in 15 chapters, it covers more than 135 experiments ranging from preparation of culture media to PCR, Southern and Western blotting, and a general introductory chapter. Every chapter is preceded with a brief theoretical background, and each experiment with principle/working and requirements. 
  • Appendices include standard values, preparation of buffer solutions, vitamin levels, etc.
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