Contents: UNITS Introduction to Biotechnology 
Cytology
Growth
Nutrition 
 Carbohydrates
Lipids
Amino Acids and Proteins 
Nucleic Acids 
Vitamins
Enzyme
Hormones and Phytohormones
Biophysical Chemistry
Microscopy
Staining
Cell Measurements
Isolation and Cultivation Techniques
Sterilization and Disinfection
Methods in Microbial Taxonomy
EXPERIMENTS / Safety in Laboratory
Preparation of Standard Solutions
Specific Gravity Determination /
Viscosity Measurement /
pH Meter
Beer's and Lambert's Law 
Paper Chromatography
Thin Layer Chromatography
Qualitative Tests for Carbohydrates
Qualitative Tests for Proteins
Amino Acids / Determination of pH Value
Isolation of Starch from Potato 
Isolation of Casein from Milk 
Estimation of Protein (Biuret Assay)
Estimation of Sugar (DNSA Method)
Determination of Iodine Number
Estimation of Acid Number 
Estimation of DNA (DPA Method) /
Estimation of RNA (Orcinol Method)
Enzyme Assay (Amylase) / Compound Microscope
Culture Media Preparation
Negative Staining (Relief Staining)
Monochrome Staining
Gram Staining
Streak Plate Technique
Pour Plate Technique
Most Probable Number (MPN)
Separation of Serum from Blood
Blood Group
Antigen-Antibody Reaction
Widal Test
Isolation of Genomic DNA 
 Isolation of Plasmid DNASDS-Page
Agarose Gel Electrophoresis
References 
Index

• The subject matter is divided in two parts; part one forms the theoretical foundation covering the broad spectrum of biological dimensions with biotechnological exploration, and part two includes the experiments to support the theory.
• Structure, mode of action and biological significance of the topics covered has been elaborated appropriately. 
• Abundantly illustrated with figures and tables for better understanding of scientific principles and skillful execution of experiments.
• The 36 experiments includes principle as well as procedure; relevant calculations, and result and interpretation.