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Mammalian Cell Technology and Its Applications
Mammalian Cell Technology and Its Applications

Tags: Mammalian Cell Technology and Its Applications, Asok Mukhopadhyay, Life Sciences, Biotechnology

Mammalian Cell Technology and Its Applications

Mammalian Cell Technology and Its Applications has been thoroughly revised keeping in view the developments in the recent past in the field of 'Regenerative Medicine'. The book is now divided into five sections consisting of sixteen chapters, which now appears to be most convenient for the diverse backgrounds of students starting from basic biology to the bioengineering discipline. Since, animal cell technology commands proper understanding of cell biology, DNA technology, immunology and bioengineering, the goal of this book is to amalgamate knowledge from these fields and pass these on to the readers who intend to start their professional career in academics or in industrial research. An animal cell is a unique factory, where thousands of genes are encoded and transcribed. Products are translated, and finally processed to biologically active molecules. It is therefore, important to understand the inside of a cell, how cellular functions are coordinated, what are the limitations of a cell, and reasons for proliferation and cellular death. In this revised version, the first two chapters are dedicated to basic cell biology aspects, which will orient the students from other backgrounds. The second section covers basic cell culture technology, which consists of preservation and control of cell lines, cell culture media, good manufacturing practices, cell culture equipment, culture environment and methods, etc. In the third section, recombinant therapeutic proteins, large-scale cell culture, and scale-up processes are discussed. The fourth section has been thoroughly revised providing detailed aspects of cell-based therapeutic applications for human (animal) diseases. The first part of this section covers basic aspects of different 'stem cells' and how they are derived. The next two chapters are dedicated to 'regenerative medicine' where tissue engineering and cell therapy approaches have been elucidated. The last section of the book deals with the analytical techniques used in cell culture practices and protocols for isolation of cells, etc.
Section I: Introduction to Cell Biology
1. Organization of Animal Cells and Special Properties
2. Proliferation and Death of Animal Cells: Molecular Mechanism
Section II: Basic Cell Culture Technology
3. Sources of Animal Cells, Preservation and Control of Cell
4. Design of Cell Culture Media
5. Good Manufacturing Practices, Aseptic Techniques, and Cell Culture Equipment
6. Environmental Factors and Cell Culture Methods
7. Separation of Viable Cells Section III: Therapeutic Proteins and Bioengineering Aspects
8. Stable Gene Expression in Mammalian Cells and Monoclonal Antibody
9. Large-scale Animal Cell Culture for the Production of Therapeutic Proteins
10. Scale-Up Studies in Animal Cell Culture Bioreactors
Section IV: Advanced Cell Culture Technology
11. Embryonic and Adult Stem Cells
12. Regenerative Medicine: Tissue Engineering
13. Regenerative Medicine: Cellular Therapy
14. Present Trend and Future Perspective
Section V: Analytical Techniques and Cell Isolation
15. Quantitative Analysis
16. Establishing Primary Cultures and Cryopreservation of Cells/Tissues Glossary
Index
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