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Molecular Biology
Molecular Biology

Tags: Molecular Biology, A Laboratory Manual, Life Sciences, Botany, Ashwani Kumar, S.K. Gakhar, Monika Miglani

Molecular Biology

The field of molecular biology continues to be the most exciting and dynamic area of science and is predicted to dominate the 21st century. Only at the molecular level, it is possible to understand various biological processes in detail. Such understanding is vital for advances in medicine, and the pharmaceutical industry that produces new drugs and thus cure is greatly dependent upon molecular biology. Molecular biology also contributes to our understanding of what human beings are and how they fit into this universe. The objective of this manual is to train young teachers from colleges and research institutions so that methodologies could be used by them in their research in various fields of biology. This would also help all the beginners and students at B.Sc. & M.Sc. level to learn the various techniques on molecular biology. This book contains four chapters giving the step-wise protocols for various practicals. In addition, it has general instructions for safety procedures, viz., biosafety, radiation safety, hazardous chemicals, personal protection and also the preparation of various stock and working solutions for molecular biology practicals.

1.Nucleic Acids
2.Proteins
3.Bioinformatics
4.General Laboratory Instructions
5.Cautions
Appendix
Index.

  • This laboratory manual explains the techniques that are used to understand the complex macromolecules in molecular biology. 
  • Spread in four chapters, it gives step-wise treatment of various practical techniques, with annotations at appropriate places. 
  • Various techniques ranging from plasmid DNA isolation to PCR amplification, techniques of DNA polymorphism analysis and hybridization protocols for DNA and RNA blot analysis have been covered in detail.
  • One dedicated chapter on general laboratory instructions comprising safety procedures, general & personal precautions, etc has been included. 
  • Provides David's Life Chart II, conversion factors, and Nucleases and Inhibitors in the appendix.

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