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Plant Biotechnology
Plant Biotechnology

Tags: Plant Biotechnology, Practical Manual, Life Sciences, Agriculture, C.C. Giri, Archana Giri

Plant Biotechnology

Plant Biotechnology: Practical Manual covers most of the important areas of present-day plant biotechnology, beginning from plant tissue culture media preparation to transgenic plant production and related molecular biology protocols. It is meant for both students who are being introduced to plant biotechnology and those wanting to do advanced research in this field. It would also be helpful for teachers to formulate their own practical protocols using different model plant systems. This book includes the principles, theoretical background and the basis for each protocol supported by the author's own research findings. This approach has been adopted to help the learners and researchers modify their procedures to develop their own protocols and methods utilizing the proven protocols included in the book.

1. Introduction to Plant Tissue Culture
2. Plant Regeneration via Organogenesis and Somatic Embryogenesis
3. Protoplast Isolation, Culture, and Fusion 
4. Encapsulation Techniques for Artificial Seeds (Synseeds)
5. Another culture and Production of Haploids
6. Production of Secondary Metabolites Using Cell Suspension Culture, Immobilized Cell
    Systems and Precursor Feeding Methods
7. Induction of Transformed Hairy Roots using Wild Strains of Agrobacterium rhizogenes,
    and Production of Secondary Metabolites 
8. Induction of Transformed Shooty Teratomas Using Specialized Strains of Agrobacterium
     turnefaciens and Production of Secondary Metabolites
9. Agrobacterium Mediated Genetic Transformation 
10.  Anatomical and Histological Techniques for Plant Tissue Cultured in vitro 
11. Scanning and Transmission Electron Microscopic Techniques for in vitro Cultured Plant
      Tissue
12. Chromosomal Analysis of in vitro Cultured Tissue and Regenerated Plants
13. Techniques for Isolation and Estimation of DNA and RNA
14. Safety Practices and Essentials in the Laboratory.

  •  Contains lab experiments in various areas of plant biotechnology.
  • Each experiment includes the principles and theoretical background covering most of the important areas of plant biotechnology. 
  • Each chapter includes end-of-the-chapter questions along with references. 
  • Each protocol is supported by author's own research findings.
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