Plant Tissue Culture
Plant Tissue Culture

Tags: Plant Tissue Culture, Life Sciences, Agriculture, B N Sathyanarayana, Dalia B. Verghese

Plant Tissue Culture

Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.
  • Historical Developments in Plant Tissue and Cell Culture
  • Laboratory Design and Development
  • Instrumentation
  • Plant Tissue Culture Media
  • Sterilization Techniques
  • Safety Rules and Regulations
  • Applications of Plant Tissue Culture
  • Micropropagation
  • Micropropagation by Proliferation of Auxillary Buds
  • Micropropagation by Adventitious Shoot Regeneration
  • Plant Regeneration from Callus Cultures
  • Plant Regeneration by Somatic Embryogenesis
  • Meristem Culture
  • In vitro Haploid Production
  • Embryo Culture
  • Endosperm Culture
  • Culturing of Roots in vitro
  • Protoplast Isolation and Culture
  • Protoplast Fusion and Cybridization
  • Storage Organ Culture
  • In vitro Germplasm Conservation
  • In vitro Production of Plant Secondary Metabolites
  • Acclimatization of Micropropagated Plantlets
  • In vitro and ex vitro Rooting of Micro Shoots
  • Somaclonal and Gametoclonal Variations
  • Agrobacterium Mediated Transformation
  • Problems in Plant Tissue Culture
  • Commercial Micropropagation
  • Microscopic and Photographic Techniques for Plant Tissue Culture
  • Histological Techniques
  • Glossary
  • Appendix
  • Selected Bibliography Readership: Graduate, Postgraduate and Research Students of Plant Tissue Culture, Biotechnology, Botany, Microbiology, Biochemistry, Agriculture, Horticulture and Allied Science
• Chapters deals with various technologies in plat tissue culture like meristem culture, axillary branching, protoplast culture and haploid production, and with the technological aspects.
• The book focuses on practical procedures and protocols of tissue culture.
• A detailed glossary, and the formula for converting molar solutions to milligrams/litre and vice versa is provides at the end of the book.
• A lot of colour pictures and line diagrams have been included in the book."


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