"“This is a very good student-friendly manual and I strongly recommend for its publication. I praise the efforts of the authors for bringing out such a manual for which nobody thought earlier.”
— Dr. Jagat Kumar Roy
Cytogenetics Lab, Banaras Hindu University, India
“This manual combines the study of the most crucial theoretical aspects in Drosophila genetics, discussing a wide spectrum of methods used in the field. Overall, the book is written in a very clear way. It has a consistent and clear structure and the figures and pictures presented are of good quality. I strongly recommend and support the publication of this manual.”
— Dr. Hector Herranz
Associate Professor, Department of Cellular and
Molecular Medicine, University of Copenhagen, Denmark
Genetics is taught in all life science related disciplines at the undergraduate level. This laboratory manual meant for beginners is an outcome of the authors’ attempt made in the last three years (2012-15) of designing experiments to teach the principles of transmission genetics using Drosophila as a model system. The purpose of Drosophila Resource Centre was to demonstrate the ease of learning genetics through experimentation. Wherever required the existing approaches have also complemented the new effort.
The experiments are designed to teach the basic principles of genetics in a one-semester course. More than 90% of the exercises were conducted using a handful of Drosophila mutants. Most of the exercises are organized in a similar fashion covering 6-8 pages. Each exercise provides background information with supporting examples from literature; protocol; observations and calculations (if any) and finally results and discussion.
• It will bridge the existing gap generally faced in teaching genetics.
• It is user friendly. Students can independently initiate crosses, analyze and interpret the underlying genetic principle.
• The manual will serve as a starting point for those who wish to teach genetics through experimentation.
• Each exercise discusses actual data generated by students in the past three years.
1. Maintenance and culturing of Drosophila
2. Sexing and collection of virgin females
3. To study the life cycle of Drosophila melanogaster
4. To study mutant phenotypes of Drosophila melanogaster
5. To demonstrate Mendel’s law of segregation and dominance
6. To demonstrate Mendel’s law of independent assortment
7. To study the inheritance of white eye colour in Drosophila
8. To map sepia and curl mutants by recombination mapping
9. To demonstrate absence of crossing over in Drosophila males
10. To establish dominance relationship between multiple alleles of a gene
11. To demonstrate that the white-eyed flies have a deposition defect
12. To perform cis-trans test of allelism with the given eye colour mutants
13. To demonstrate dosage compensation in Drosophila melanogaster
14. To create a Bar and apricot eyed double mutant
15. To detect recessive lethal alleles in genetic crosses
16. To study the penetrance and expressivity of serrate and vestigial wings in Drosophila melanogas
17. To study the characteristics of X-linked dominant traits
18. To separate eye pigments (pterins) by thin layer chromatography
19. To prepare stained squashes of salivary glands showing well spread polytene chromosomes
20. To demonstrate recombination within a gene
21. To determine the inheritance pattern of white eye colour and vestigial wings from the F2 data
22. To dissect the malphigian tubules, brain and imaginal discs from the third instar Drosophila larva
23. To analyse using bioinformatic tools Drosophila as a model system (Toll 9 gene) for human diseases (asthma)
24. To test for PTC tasting in a random population and to calculate allele and genotype frequency using Hardy Weinberg law
25. To determine the ABO blood groups in a random sample and calculate the allele frequency using Hardy Weinberg law